build: PyInstaller打包配置+启动器

web/app.py

@@ -2,6 +2,7 @@
 cDNA微阵列图像处理 - Web UI (Flask)
 =====================================
 启动：python web/app.py
+打包：python build_exe.py
 打开：http://localhost:5000
 """
 
@@ -19,7 +20,16 @@ from scipy import ndimage
 BASE_DIR = os.path.dirname(os.path.dirname(os.path.abspath(__file__)))
 sys.path.insert(0, os.path.join(BASE_DIR, 'src'))
 
-app = Flask(__name__)
+# PyInstaller 打包后资源路径
+if getattr(sys, 'frozen', False):
+    bundle_dir = sys._MEIPASS
+    template_dir = os.path.join(bundle_dir, 'templates')
+    static_dir = os.path.join(bundle_dir, 'static')
+else:
+    template_dir = os.path.join(os.path.dirname(os.path.abspath(__file__)), 'templates')
+    static_dir = os.path.join(os.path.dirname(os.path.abspath(__file__)), 'static')
+
+app = Flask(__name__, template_folder=template_dir, static_folder=static_dir)
 app.config['MAX_CONTENT_LENGTH'] = 50 * 1024 * 1024  # 50MB
 UPLOAD_DIR = os.path.join(os.path.dirname(os.path.abspath(__file__)), 'uploads')
 os.makedirs(UPLOAD_DIR, exist_ok=True)

web/launcher.py

@@ -0,0 +1,22 @@
+"""cDNA Analyzer 启动器 — 供 PyInstaller 打包"""
+import os, sys, threading
+
+# PyInstaller 打包后资源路径
+if getattr(sys, 'frozen', False):
+    base = sys._MEIPASS
+else:
+    base = os.path.dirname(os.path.dirname(os.path.abspath(__file__)))
+
+# 设置模板和静态文件目录
+os.environ['TEMPLATE_DIR'] = os.path.join(base, 'templates')
+os.environ['STATIC_DIR'] = os.path.join(base, 'static')
+
+# 导入 Flask app
+from app import app
+
+def open_browser():
+    os.startfile('http://localhost:5000')
+
+if __name__ == '__main__':
+    threading.Timer(1.5, open_browser).start()
+    app.run(debug=False, port=5000)