build: PyInstaller打包配置+启动器

build_exe.py

@@ -0,0 +1,26 @@
+"""
+PyInstaller打包脚本
+运行: python build_exe.py
+输出: dist/cDNA_Analyzer.exe
+"""
+
+import PyInstaller.__main__
+import os
+
+BASE = os.path.dirname(os.path.abspath(__file__))
+
+PyInstaller.__main__.run([
+    'web/launcher.py',
+    '--name=cDNA_Analyzer',
+    '--onefile',
+    '--windowed',
+    '--add-data', f'web/templates;templates',
+    '--add-data', f'web/static;static',
+    '--hidden-import', 'skimage',
+    '--hidden-import', 'scipy.ndimage',
+    '--hidden-import', 'matplotlib.backends.backend_agg',
+    '--collect-all', 'skimage',
+    '--noconfirm',
+    '--clean',
+])
+print('\n完成！exe 在 dist/ 目录')

cDNA_Analyzer.spec

@@ -0,0 +1,45 @@
+# -*- mode: python ; coding: utf-8 -*-
+from PyInstaller.utils.hooks import collect_all
+
+datas = [('web/templates', 'templates'), ('web/static', 'static')]
+binaries = []
+hiddenimports = ['skimage', 'scipy.ndimage', 'matplotlib.backends.backend_agg']
+tmp_ret = collect_all('skimage')
+datas += tmp_ret[0]; binaries += tmp_ret[1]; hiddenimports += tmp_ret[2]
+
+
+a = Analysis(
+    ['web\\launcher.py'],
+    pathex=[],
+    binaries=binaries,
+    datas=datas,
+    hiddenimports=hiddenimports,
+    hookspath=[],
+    hooksconfig={},
+    runtime_hooks=[],
+    excludes=[],
+    noarchive=False,
+    optimize=0,
+)
+pyz = PYZ(a.pure)
+
+exe = EXE(
+    pyz,
+    a.scripts,
+    a.binaries,
+    a.datas,
+    [],
+    name='cDNA_Analyzer',
+    debug=False,
+    bootloader_ignore_signals=False,
+    strip=False,
+    upx=True,
+    upx_exclude=[],
+    runtime_tmpdir=None,
+    console=False,
+    disable_windowed_traceback=False,
+    argv_emulation=False,
+    target_arch=None,
+    codesign_identity=None,
+    entitlements_file=None,
+)

web/app.py

@@ -2,6 +2,7 @@
 cDNA微阵列图像处理 - Web UI (Flask)
 =====================================
 启动：python web/app.py
+打包：python build_exe.py
 打开：http://localhost:5000
 """
 
@@ -19,7 +20,16 @@ from scipy import ndimage
 BASE_DIR = os.path.dirname(os.path.dirname(os.path.abspath(__file__)))
 sys.path.insert(0, os.path.join(BASE_DIR, 'src'))
 
-app = Flask(__name__)
+# PyInstaller 打包后资源路径
+if getattr(sys, 'frozen', False):
+    bundle_dir = sys._MEIPASS
+    template_dir = os.path.join(bundle_dir, 'templates')
+    static_dir = os.path.join(bundle_dir, 'static')
+else:
+    template_dir = os.path.join(os.path.dirname(os.path.abspath(__file__)), 'templates')
+    static_dir = os.path.join(os.path.dirname(os.path.abspath(__file__)), 'static')
+
+app = Flask(__name__, template_folder=template_dir, static_folder=static_dir)
 app.config['MAX_CONTENT_LENGTH'] = 50 * 1024 * 1024  # 50MB
 UPLOAD_DIR = os.path.join(os.path.dirname(os.path.abspath(__file__)), 'uploads')
 os.makedirs(UPLOAD_DIR, exist_ok=True)

web/launcher.py

@@ -0,0 +1,22 @@
+"""cDNA Analyzer 启动器 — 供 PyInstaller 打包"""
+import os, sys, threading
+
+# PyInstaller 打包后资源路径
+if getattr(sys, 'frozen', False):
+    base = sys._MEIPASS
+else:
+    base = os.path.dirname(os.path.dirname(os.path.abspath(__file__)))
+
+# 设置模板和静态文件目录
+os.environ['TEMPLATE_DIR'] = os.path.join(base, 'templates')
+os.environ['STATIC_DIR'] = os.path.join(base, 'static')
+
+# 导入 Flask app
+from app import app
+
+def open_browser():
+    os.startfile('http://localhost:5000')
+
+if __name__ == '__main__':
+    threading.Timer(1.5, open_browser).start()
+    app.run(debug=False, port=5000)