feat: cDNA微阵列图像处理作业 - Python实现

实现内容： - 网格划分：投影分析 + 自相关估周期 + 白顶帽去背景 + 质心提取 - 三种阈值分割：人工阈值、Otsu自动阈值、迭代阈值 - TV去噪（Chambolle投影算法） - 后处理：去小连通域 + 保留最大连通域 - 完整可视化：网格叠加、阈值对比、收敛曲线、分割结果参考MATLAB代码：NewGridAndCV/demo_GriddingAndCV.m
2026-05-06 19:41:26 +08:00
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+
+function m = maskcircle2(I,type)
+% auto pick a circular mask for image I 
+% built-in mask creation function
+% Input: I   : input image     
+%        type: mask shape keywords
+% Output: m  : mask image
+
+% Copyright (c) 2009, 
+% Yue Wu @ ECE Department, Tufts University
+% All Rights Reserved  
+
+if size(I,3)~=3
+    temp = double(I(:,:,1));
+else
+    temp = double(rgb2gray(I));
+end
+
+h = [0 1 0; 1 -4 1; 0 1 0];
+T = conv2(temp,h);
+T(1,:) = 0;
+T(end,:) = 0;
+T(:,1) = 0;
+T(:,end) = 0;
+
+thre = max(max(abs(T)))*.5;
+idx = find(abs(T) > thre);
+[cx,cy] = ind2sub(size(T),idx);
+cx = round(mean(cx));
+cy = round(mean(cy));
+
+[x,y] = meshgrid(1:min(size(temp,1),size(temp,2)));
+
+m = zeros(size(temp));
+[p,q] = size(temp);
+
+switch lower (type)
+    case 'small'
+        r = 10;
+        n = zeros(size(x));
+        n((x-cx).^2+(y-cy).^2<r.^2) = 1;
+        m(1:size(n,1),1:size(n,2)) = n;
+        %m((x-cx).^2+(y-cy).^2<r.^2) = 1;
+    case 'medium'
+        r = min(min(cx,p-cx),min(cy,q-cy));
+        r = max(2/3*r,25);
+        n = zeros(size(x));
+        n((x-cx).^2+(y-cy).^2<r.^2) = 1;
+        m(1:size(n,1),1:size(n,2)) = n;
+        %m((x-cx).^2+(y-cy).^2<r.^2) = 1;
+    case 'large'
+        r = min(min(cx,p-cx),min(cy,q-cy));
+        r = max(2/3*r,60);
+        n = zeros(size(x));
+        n((x-cx).^2+(y-cy).^2<r.^2) = 1;
+        m(1:size(n,1),1:size(n,2)) = n;
+        %m((x-cx).^2+(y-cy).^2<r.^2) = 1;
+    case 'whole'
+        r = 9;
+        m = zeros(round(ceil(max(p,q)/2/(r+1))*3*(r+1)));
+        siz = size(m,1);
+        sx = round(siz/2);       
+        i = 1:round(siz/2/(r+1));
+        j = 1:round(0.9*siz/2/(r+1));
+        j = j-round(median(j)); 
+        m(sx+2*j*(r+1),(2*i-1)*(r+1)) = 1;
+        se = strel('disk',r);
+        m = imdilate(m,se);
+        m = m(round(siz/2-p/2-6):round(siz/2-p/2-6)+p-1,round(siz/2-q/2-6):round(siz/2-q/2-6)+q-1); 
+end
+        tem(:,:,1) = m;
+        M = padarray(m,[floor(2/3*r),floor(2/3*r)],0,'post');
+        tem(:,:,2) = M(floor(2/3*r)+1:end,floor(2/3*r)+1:end);
+        m = tem; 
+return